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( A ) The appearance of Asaia colonies on the glucose–yeast extract–CaCO3 medium. Four types of colonies were obtained after culturing, with the colony ranging in colors from pink to yellowish, white, and smooth. Asaia species produce white medium noninvasive ( a ), white large invasive ( b ), yellow smooth ( c ), and bright pink ( d ) colonies on the glucose–yease extract–CaCO3 medium, which is one of their unique morphological characteristics. ( B ) Evolutionary analysis of the isolated Asaia bacteria. A phylogenetic tree was created with MEGAX software and <t>ClustalW</t> program based on 16S rRNA gene sequences. The maximum likelihood method was used to infer evolutionary relationships, and associated taxa were clustered together in the bootstrap test (1,000 replicates). The red-colored branches represent the sequences of the different isolated Asaia colonies according to their appearnce, while the black branches represent the previously described sequences of Asaia.
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( A ) The appearance of Asaia colonies on the glucose–yeast extract–CaCO3 medium. Four types of colonies were obtained after culturing, with the colony ranging in colors from pink to yellowish, white, and smooth. Asaia species produce white medium noninvasive ( a ), white large invasive ( b ), yellow smooth ( c ), and bright pink ( d ) colonies on the glucose–yease extract–CaCO3 medium, which is one of their unique morphological characteristics. ( B ) Evolutionary analysis of the isolated Asaia bacteria. A phylogenetic tree was created with MEGAX software and <t>ClustalW</t> program based on 16S rRNA gene sequences. The maximum likelihood method was used to infer evolutionary relationships, and associated taxa were clustered together in the bootstrap test (1,000 replicates). The red-colored branches represent the sequences of the different isolated Asaia colonies according to their appearnce, while the black branches represent the previously described sequences of Asaia.
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multi-alignment tool clustalw - by Bioz Stars, 2026-04
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( A ) The appearance of Asaia colonies on the glucose–yeast extract–CaCO3 medium. Four types of colonies were obtained after culturing, with the colony ranging in colors from pink to yellowish, white, and smooth. Asaia species produce white medium noninvasive ( a ), white large invasive ( b ), yellow smooth ( c ), and bright pink ( d ) colonies on the glucose–yease extract–CaCO3 medium, which is one of their unique morphological characteristics. ( B ) Evolutionary analysis of the isolated Asaia bacteria. A phylogenetic tree was created with MEGAX software and ClustalW program based on 16S rRNA gene sequences. The maximum likelihood method was used to infer evolutionary relationships, and associated taxa were clustered together in the bootstrap test (1,000 replicates). The red-colored branches represent the sequences of the different isolated Asaia colonies according to their appearnce, while the black branches represent the previously described sequences of Asaia.

Journal: Microbiology Spectrum

Article Title: Contrasting patterns of Asaia association with Plasmodium falciparum between field-collected Anopheles gambiae and Anopheles coluzzii from Cameroon

doi: 10.1128/spectrum.00567-24

Figure Lengend Snippet: ( A ) The appearance of Asaia colonies on the glucose–yeast extract–CaCO3 medium. Four types of colonies were obtained after culturing, with the colony ranging in colors from pink to yellowish, white, and smooth. Asaia species produce white medium noninvasive ( a ), white large invasive ( b ), yellow smooth ( c ), and bright pink ( d ) colonies on the glucose–yease extract–CaCO3 medium, which is one of their unique morphological characteristics. ( B ) Evolutionary analysis of the isolated Asaia bacteria. A phylogenetic tree was created with MEGAX software and ClustalW program based on 16S rRNA gene sequences. The maximum likelihood method was used to infer evolutionary relationships, and associated taxa were clustered together in the bootstrap test (1,000 replicates). The red-colored branches represent the sequences of the different isolated Asaia colonies according to their appearnce, while the black branches represent the previously described sequences of Asaia.

Article Snippet: These sequences were aligned with previous existing isolates using the ClustalW multisequence alignment tool in BioEdit software.

Techniques: Isolation, Bacteria, Software